概述
-
货号
YVV15501
-
描述
Lambda Protein Phosphatase (Lambda PP) is a Mn2+-dependent protein phosphatase with activity towards phosphorylated serine, threonine and tyrosine residues. One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 minute at 30°C in a total reaction volume of 50 µl.
-
表达系统
E. coli -
种属
Escherichia phage lambda (Bacteriophage lambda) -
蛋白长度
Lambda PP is cloned from Escherichia phage lambda and expressed in E.coli. -
预测分子量
27.32 kDa -
性质
Recombinant -
靶标
Lambda Protein Phosphatase (Lambda PP) -
浓度
370U/μl -
内毒素水平
Please contact with the lab for this information. -
纯度
>90% as determined by SDS-PAGE. -
应用
Remove phosphate groups from phosphorylated serine,threonine and tyrosine residues in proteins. -
状态
Liquid -
保存溶液
100 mM NaCl, 50 mM HEPES, pH 7.5, 0.1 mM MnCl2, 0.1 mM EDTA, 2 mM DTT, 0.01% Brij35, and 50% glycerol. -
精准度
Reaction Condition
1X Buffer for Lambda PP
Supplement with 1 mM MnCl2
Incubate at 30°CProtocol
1. Combine protein sample and H2O (if necessary) to a total volume of 38 µl.
2. Add 5 μL of 10X Buffer for Lambda PP and 5 μL of 10 mM MnCl2 to make a total reaction volume of 50 µl.
3. Add 2 µl of Lambda PP.
4. Incubate at 30°C for 30 minutes. -
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. -
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
图片
参考文献
评价
