概述
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货号
YXX05201
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描述
Endo S is an endoglycosidase specific for cleaving the N-linked glycans from the chitobiose core of the heavy chain of native IgG.
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生物活性
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 5 μg of native mouse monoclonal IgG in 1 hour at 37°C in a total reaction volume of 10 µl. -
表达系统
E. coli -
种属
Streptococcus pyogenes -
蛋白长度
EndoS is cloned from Streptococcus pyogenes and expressed in E.coli. -
预测分子量
109.35 kDa -
性质
Recombinant -
靶标
Endo S -
浓度
40000U/ml -
内毒素水平
Please contact with the lab for this information. -
纯度
>95% as determined by SDS-PAGE. -
应用
Expression Systems,Proteomics,Glycan Sequencing,Recombinant Glycoprotein Expression,Glycoprotein Analysis -
状态
Liquid -
保存溶液
0.01M PBS, pH 7.4, 50% glycerol -
实验操作流程
Protocol
Digestion reaction condition
Reagent
Volume
Native IgG concentration(μg/μl)
C
Native IgG volume (μl)
V
Native IgG quality (μg)
100
Endo S(U)
20
Endo S(μl)
0.5
10X buffer(μl）
200
ddH2O
(200*10)-0.5-V
Reaction system(μl)
200*10
Incubate reaction at 37°C for 1 hour.
1X Buffer
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C) -
运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. -
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
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