IdeS Protease (IgG specific) (YXX05001)

One unit will cleave ≥95% of 1μg of recombinant monoclonal IgG in 30 minutes at 37°C.

E. coli

Streptococcus pyogenes

IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.

37.57 kDa

Recombinant

IdeS Protease (IgG Specific)

40000U/ml

Please contact with the lab for this information.

>90% as determined by SDS-PAGE.

Proteomics

Liquid

0.01M PBS, pH 7.4, 50% glycerol

Digestion reaction condition

37℃, 30min

 

Protocol

1. Add appropriate amount of IgG (to 5mg) in digestive juice;

2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG;

3. Incubate the sample at 37°C for 30-60min.

* IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.