概述
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货号
EBF78301
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描述
Enterokinase is a specific protease that cleaves after lysine at its cleavage site Asp-Asp-Asp-Asp-Lys. It will sometimes cleave at other basic residues, depending on the conformation of the protein substrate.
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生物活性
At 25 ° C, reaction buffer 50 mM Tris-HCl (PH 8.0), the amount of enzyme required to digest 50 ug fusion protein containing the digestion site within 16 hours is defined as 1 unit of enzyme activity, which is 1U. -
表达系统
Yeast -
种属
Bovine -
蛋白长度
Enterokinase is cloned from bovine and expressed in Yeast. -
预测分子量
22.11 kDa -
性质
Recombinant -
靶标
Enterokinase -
浓度
> 30 KU / mg, 1mg of recombinant bovine enterokinase can cut at least 1.5g fusion protein. -
内毒素水平
Please contact with the lab for this information. -
纯度
>90% as determined by SDS-PAGE. -
应用
Fusion Protein Cleavage,Protein Purification,Protein Digestion -
状态
Liquid -
保存溶液
50 mM Tris-HCl, 50%Glycerol, pH 8.0 -
实验操作流程
Digestion reaction condition
Digestion at 25 ℃ for 16 hours
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运输
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. -
稳定性和存储
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
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