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货号
KAD76501
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描述
This assay employs the quantitative sandwich enzyme immunoassay technique. Romiplostim has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and anti-Romiplostim will be captured by immobilized Romiplostim. After washing away any unbound substances, a biotin-labeled Romiplostim is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Romiplostim bound in the initial step. The color development is stopped and the intensity of the color is measured.
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应用
Used for the quantitative determination of Anti-Romiplostim concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
31.25 - 2,000 ng/mL -
灵敏度
17.08 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
848.7
206.8
46.8
888.7
225.1
53.9
Standard deviation
24.6
9.6
4.3
34.7
13.0
2.8
CV (%)
2.9
4.7
9.1
3.9
5.8
5.2
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
