-
货号
KAK13901
-
描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Semaglutide has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-Semaglutide present is bound by the immobilized protein. After washing away any unbound substances, a HRP conjugated probe is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Semaglutide bound in the initial step. The color development is stopped and the intensity of the color is measured.
-
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
15.63 - 10,000 ng/mL -
灵敏度
6.49 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
519.9
135.2
30.9
542.8
112.8
17.3
Standard deviation
28.3
9.5
2.0
71.5
16.7
2.5
CV (%)
5.5
7.1
6.4
13.2
14.8
14.7
-
回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃. -
别名
CAS, 910463-68-2, Semaglutide, Rybelsus, Ozempic, NN9535, OG217SC, NNC 0113-0217
