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货号
KAV13102
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant MPXV A35R antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human Anti-MPXV A35R IgG present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgG antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human Anti-MPXV A35R IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
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靶标
A35R -
应用
Used for the quantitative determination of Human Anti-MPXV A35R IgG concentration in serum and plasma. -
检测方法
Colorimetric -
样本类型
Plasma, Serum -
实验类型
Quantitative -
检测范围
0.625 - 20 ng/mL -
灵敏度
0.62 ng/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (ng/mL)
9.6
2.5
1.3
11.3
2.9
0.8
Standard deviation
0.4
0.1
0.0
0.4
0.2
0.1
CV (%)
4.0
2.4
2.8
3.6
5.5
10.4
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
