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货号
KVV00301
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描述
PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for SARS-CoV-2 RBD has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any SARS-CoV-2 RBD present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled SARS-CoV-2 RBD antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells.Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SARS-CoV-2 RBD bound in the initial step. The color development is stopped and the intensity of the color is measured.
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靶标
RBD -
应用
Used for the quantitative determination of SARS-CoV-2 RBD concentration in serum, plasma, bronchoalveolar lavage fluid and nasopharyngeal swab samples. -
检测方法
Colorimetric -
样本类型
Serum, Plasma and Cell culture supernatant. -
实验类型
Quantitative -
检测范围
12.5 - 800 pg/mL -
灵敏度
4.92 pg/mL -
精准度
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
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回收率
80-120% -
运输
2-8 ℃ -
稳定性和存储
When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20℃, the rest reagents should be store at 4℃.
